New analytical and diagnostic methods in infectious diseases and tropical medicine
The focus of the working group is on the development of new techniques for investigating bacterial and viral pathogenicity and the efficacy of antimicrobial/viral substances. The research and development work focuses on the detection of metabolic activity of (individual) organisms under therapy. Here, mainly mass spectrometry-based techniques (e.g. MALDI-TOF-MS, LC-MS/MS in combination with stable isotope labeling), spectroscopic methods (e.g. RAMAN), microscopic methods (e.g. fluorescence), microfluidic-based techniques, genetic engineering techniques (vector design, GMO), arthropods/vectors - new techniques in vector biology; serological techniques and methods for immunomonitoring of SARS-CoV-2 infections.
The research group also developed a wastewater monitoring protocol, which has been implemented since April 2020 and determines the load of SARS-CoV-2 viruses in wastewater at 6 different points in the Munich wastewater network on a weekly basis (for more information, see Projects).
Ongoing projects
In the "DynamicKit" research project as part of the BayResQnet research network, the turnover kinetics of the proteins of mycobacteria are being investigated and analyzed using artificial intelligence (AI)-based algorithms. The project is being carried out in cooperation with Helmholtz Munich and the microbiology department of the Max von Pettenkofer Institute.
As part of the fight against the SARS-CoV-2 pandemic, a new laboratory section was set up that primarily deals with immuno-monitoring after COVID-19 or corresponding vaccinations. A wide range of tests were established and evaluated in cooperation with external partners and companies. In addition, a method for measuring SARS-CoV-2 titers from dried blood spots (DBS) was established, which allows home testing for antibodies with high reliability.
The research group developed a wastewater monitoring protocol that has been implemented since April 2020 and determines the load of SARS-CoV-2 viruses in wastewater at 6 different points in Munich's wastewater network on a weekly basis. This made it possible to track the pandemic. In cooperation with the virology department of the Max von Pettenkofer Institute, the analyses were further refined and also by means of sequencing in cooperation with the LMU Gene Center (see also cooperation partners/funding).
Bay-VOC - SARS-CoV-2 wastewater monitoring in Bavaria:
The map of the Bay-VOC initiative shows an overview of the viral load in wastewater in Bavaria.
Messages about the project
The project is also funded by the pan-European consortium ORCHESTRA. The ORCHESTRA project has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No 101016167. This publication reflects the views only of the author, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
Funding includes funds and projects from, among others:
- German Center for Infection Research (DZIF)
- LMU research funding
- Friedrich Baur Institute
- DFG cooperation project with Prof. Dr. Haisch
- ESTHER cooperation project with Dr. med. Arne Kroidl
- BayResQNet
- Bavarian State Chancellery (SARS-CoV-2 projects)
- Horizon 2020 (ORCHESTRA)
- Bavarian State Ministry of Economic Affairs, Regional Development and Energy (Pandemic Preparedness)
- Roche
Cooperation partner wastewater monitoring project:
- Center for International Health (CIH), LMU Klinikum München
- German Center for Infection Research (DZIF), Munich site
- Munich Municipal Drainage
- Munich Fire Department
- Health Department of the City of Munich
- Task Force Infectiology, Bavarian State Office for Health and Food Safety (LGL)
- Gene Center of the LMU Munich
- Max von Pettenkofer Institute for Virology, LMU
- Helmholtz Munich, Institute of Computational Biology (ICB)
The wastewater monitoring project is also funded by the pan-European consortium ORCHESTRA. The ORCHESTRA project has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No 101016167. This publication reflects the views only of the author, and the Commission cannot be held responsible for any use which may be made of the information contained therein.
2022:
The interplay of viral loads, clinical presentation, and serological responses in SARS-CoV-2 - Results from a prospective cohort of outpatient COVID-19 cases.
Puchinger K, Castelletti N, Rubio-Acero R, Geldmacher C, Eser TM, Deák F, Paunovic I, Bakuli A, Saathoff E, von Meyer A, Markgraf A, Falk P, Reich J, Riess F, Girl P, Müller K, Radon K, Guggenbuehl Noller JM, Wölfel R, Hoelscher M, Kroidl I, Wieser A, Olbrich L; KoCo19 study group.
Virology. 2022 Apr;569:37-43. (IF ca. 3.62)
Protective immune trajectories in early viral containment of non-pneumonic SARS-CoV-2 infection.
Pekayvaz K, Leunig A, Kaiser R, Joppich M, Brambs S, Janjic A, Popp O, Nixdorf D, Fumagalli V, Schmidt N, Polewka V, Anjum A, Knottenberg V, Eivers L, Wange LE, Gold C, Kirchner M, Muenchhoff M, Hellmuth JC, Scherer C, Rubio-Acero R, Eser T, Deák F, Puchinger K, Kuhl N, Linder A, Saar K, Tomas L, Schulz C, Wieser A, Enard W, Kroidl I, Geldmacher C, von Bergwelt-Baildon M, Keppler OT, Munschauer M, Iannacone M, Zimmer R, Mertins P, Hubner N, Hoelscher M, Massberg S, Stark K, Nicolai L.
Nat Commun. 2022 Feb 23;13(1):1018. (IF ca. 14.9)
SARS-CoV-2 Beta variant infection elicits potent lineage-specific and cross-reactive antibodies.
Reincke SM, Yuan M, Kornau HC, Corman VM, van Hoof S, Sánchez-Sendin E, Ramberger M, Yu W, Hua Y, Tien H, Schmidt ML, Schwarz T, Jeworowski LM, Brandl SE, Rasmussen HF, Homeyer MA, Stöffler L, Barner M, Kunkel D, Huo S, Horler J, von Wardenburg N, Kroidl I, Eser TM, Wieser A, Geldmacher C, Hoelscher M, Gänzer H, Weiss G, Schmitz D, Drosten C, Prüss H, Wilson IA, Kreye J.
Science. 2022 Feb 18;375(6582):782-787. (IF ca. 47.7)
Diagnostic Challenges of Helicobacter pylori Infection in Ethiopia: A Community-Based Cross-Sectional Study.
Gudina EK, Amare H, Ali S, Berhane Arefayine M, Tewolde D, Tesfaye Eshete M, Jebena MG, Wieser A, Froeschl G, Tesfaye M, Desalegn H, Gashaw M.
Can J Gastroenterol Hepatol. 2022 Jun 2;2022:4013020. doi: 10.1155/2022/4013020. eCollection 2022.PMID: 36247045
Depression among people with dyspepsia and H. pylori infection: A community based cross-sectional study in Ethiopia.
Soboka M, Gudina EK, Gashaw M, Amare H, Berhane M, Desalegn H, Tewolde D, Jebena MG, Ali S, Wieser A, Froeschl G, Tesfaye M.
PLoS One. 2022 Oct 6;17(10):e0275424. doi: 10.1371/journal.pone.0275424. eCollection 2022.PMID: 36201454
International Travel as a Risk Factor for Carriage of Extended-Spectrum β-Lactamase-Producing Escherichia coli in a Large Sample of European Individuals-The AWARE Study.
Rodríguez-Molina D, Berglund F, Blaak H, Flach CF, Kemper M, Marutescu L, Pircalabioru Gradisteanu G, Popa M, Spießberger B, Wengenroth L, Chifiriuc MC, Larsson DGJ, Nowak D, Radon K, de Roda Husman AM, Wieser A, Schmitt H.
Int J Environ Res Public Health. 2022 Apr 14;19(8):4758. doi: 10.3390/ijerph19084758.PMID: 35457624
Community Wastewater-Based Surveillance Can Be a Cost-Effective Approach to Track COVID-19 Outbreak in Low-Resource Settings: Feasibility Assessment for Ethiopia Context.
Ali S, Gudina EK, Gize A, Aliy A, Adankie BT, Tsegaye W, Hundie GB, Muleta MB, Chibssa TR, Belaineh R, Negessu D, Shegu D, Froeschl G, Wieser A.
Int J Environ Res Public Health. 2022 Jul 12;19(14):8515. doi: 10.3390/ijerph19148515.PMID: 35886369
Prevalence and intensity of soil-transmitted helminth infections and associated risk factors among household heads living in the peri-urban areas of Jimma town, Oromia, Ethiopia: A community-based cross-sectional study.
Zeynudin A, Degefa T, Tesfaye M, Suleman S, Yesuf EA, Hajikelil Z, Ali S, Azam K, Husen A, Yasin J, Wieser A.
PLoS One. 2022 Sep 15;17(9):e0274702. doi: 10.1371/journal.pone.0274702. eCollection 2022.PMID: 36107925
Determinants of anti-S immune response at 6 months after COVID-19 vaccination in a multicentric European cohort of healthcare workers - ORCHESTRA project.
Collatuzzo G, Visci G, Violante FS, Porru S, Spiteri G, Monaco MGL, Larese Fillon F, Negro C, Janke C, Castelletti N, De Palma G, Sansone E, Mates D, Teodorescu S, Fabiánová E, Bérešová J, Vimercati L, Tafuri S, Abedini M, Ditano G, Asafo SS, Boffetta P; Orchestra WP5 Working Group.
Front Immunol. 2022 Sep 29;13:986085. doi: 10.3389/fimmu.2022.986085. eCollection 2022.PMID: 36248889
Surveillance of Acute SARS-CoV-2 Infections in Elementary Schools and Daycare Facilities in Bavaria, Germany (09/2020-03/2021).
Kern A, Kuhlmann PH, Matl S, Ege M, Maison N, Eckert J, von Both U, Behrends U, Anger M, Frühwald MC, Gerstlauer M, Woelfle J, Neubert A, Melter M, Liese J, Goettler D, Sing A, Liebl B, Hübner J, Klein C; COVID Kids Bavaria Consortium.
Front Pediatr. 2022 Jul 6;10:888498. doi: 10.3389/fped.2022.888498. eCollection 2022.PMID: 35874561
Enhanced spike-specific, but attenuated nucleocapsid-specific T cell responses upon SARS-CoV-2 breakthrough versus non-breakthrough infections.
Ahmed MIM, Diepers P, Janke C, Plank M, Eser TM, Rubio-Acero R, Fuchs A, Baranov O, Castelletti N, Kroidl I, Olbrich L, Bauer B, Wang D, Prelog M, Liese JG, Reinkemeyer C, Hoelscher M, Steininger P, Überla K, Wieser A, Geldmacher C.
Front Immunol. 2022 Dec 13;13:1026473. doi: 10.3389/fimmu.2022.1026473. eCollection 2022.PMID: 36582222
Distinct and dynamic activation profiles of circulating dendritic cells and monocytes in mild COVID-19 and after yellow fever vaccination.
Winheim E, Eser T, Deák F, Ahmed MIM, Baranov O, Rinke L, Eisenächer K, Santos-Peral A, Karimzadeh H, Pritsch M, Scherer C, Muenchhoff M, Hellmuth JC, von Bergwelt-Baildon M, Olbrich L, Hoelscher M, Wieser A, Kroidl I, Rothenfusser S, Geldmacher C, Krug AB.
Eur J Immunol. 2022 Nov 20. doi: 10.1002/eji.202250090. Online ahead of print.PMID: 36404054
2021:
Carriage of ESBL-producing Enterobacterales in wastewater treatment plant workers and surrounding residents - the AWARE Study.
Rodríguez-Molina D, Berglund F, Blaak H, Flach CF, Kemper M, Marutescu L, Gradisteanu GP, Popa M, Spießberger B, Weinmann T, Wengenroth L, Chifiriuc MC, Larsson DGJ, Nowak D, Radon K, de Roda Husman AM, Wieser A, Schmitt H.
Eur J Clin Microbiol Infect Dis. 2021 Dec 13:1-16. (IF ca. 3.02)
Community SARS-CoV-2 seroprevalence before and after the second wave of SARS-CoV-2 infection in Harare, Zimbabwe.
Fryatt A, Simms V, Bandason T, Redzo N, Olaru ID, Ndhlovu CE, Mujuru H, Rusakaniko S, Hoelscher M, Rubio-Acero R, Paunovic I, Wieser A, Chonzi P, Masunda K, Ferrand RA, Kranzer K.
EClinical medicine Volume 4, 101172, November 01, 2021 (IF ca. 3.5)
Seroepidemiology and model-based prediction of SARS-CoV-2 in Ethiopia: longitudinal cohort study among front-line hospital workers and communities.
Gudina EK*, Ali S*, Girma E, Gize A, Tegene B, Hundie GB, Sime WT, Ambachew R, Gebreyohanns A, Bekele M, Bakuli A, Elsbernd K, Merkt S, Contento L, Hoelscher M, Hasenauer J, Wieser A*, Kroidl A*.
LancetGlob Health. 2021 Nov;9(11):e1517-e1527 (*: shared authorships) (IF 26.7)
Spatially resolved qualified sewage spot sampling to track SARS-CoV-2 dynamics in Munich - One year of experience.
Rubio-Acero R*, Beyerl J*, Muenchhoff M, Roth MS, Castelletti N, Paunovic I, Radon K, Springer B, Nagel C, Boehm B, Böhmer MM, Graf A, Blum H, Krebs S, Keppler OT, Osterman A, Khan ZN, Hoelscher M, Wieser A; KoCo19-Study Group.
Sci Total Environ. 2021 Nov 25;797:149031. (IF 6.55)
Head-to-head evaluation of seven different seroassays including direct viral neutralization in a representative cohort for SARS-CoV-2.
Olbrich L*, Castelletti N*, Schälte Y*, Garí M*, Pütz P, Bakuli A, Pritsch M, Kroidl I, Saathoff E, Guggenbuehl Noller JM, Fingerle V, Le Gleut R, Gilberg L, Brand I, Falk P, Markgraf A, Deák F, Riess F, Diefenbach M, Eser T, Weinauer F, Martin S, Quenzel EM, Becker M, Durner J, Girl P, Müller K, Radon K, Fuchs C, Wölfel R*, Hasenauer J*, Hoelscher M*, Wieser A*; On Behalf Of The KoCo-Study Group.
JGen Virol. 2021 Oct;102(10).(*: shared authorships) (IF 3.37)
Following Pediatric and Adult IBD Patients through the COVID-19 Pandemic: Changes in Psychosocial Burden and Perception of Infection Risk and Harm over Time.
Koletzko L*, Klucker E*, Le Thi TG*, Breiteneicher S, Rubio-Acero R, Neuhaus L, Stark RG, Standl M, Wieser A, Török H, Koletzko S, Schwerd T.
JClin Med. 2021 Sep 13;10(18):4124. (*: shared authorships) (IF 4.24)
Albumin Might Attenuate Bacteria-Induced Damage on Kupffer Cells for Patients with Chronic Liver Disease.
Lin H, Fan Y, Wieser A, Zhang J, Regel I, Nieß H, Mayerle J, Gerbes AL, Steib CJ.
Cells. 2021 Sep 3;10(9):2298. (IF 6.60)
From first to second wave: follow-up of the prospective COVID-19 cohort (KoCo19) in Munich (Germany).
Radon K, Bakuli A, Pütz P, Le Gleut R, Guggenbuehl Noller JM, Olbrich L, Saathoff E, Garí M, Schälte Y, Frahnow T, Wölfel R, Pritsch M, Rothe C, Pletschette M, Rubio-Acero R, Beyerl J, Metaxa D, Forster F, Thiel V, Castelletti N, Rieß F, Diefenbach MN, Fröschl G, Bruger J, Winter S, Frese J, Puchinger K, Brand I, Kroidl I, Wieser A*, Hoelscher M*, Hasenauer J*, Fuchs C*; KoCo19 study group.
BMC Infect Dis. 2021 Sep 8;21(1):925. (*: shared authorships) (IF 3.01)
Clinical Profile of Neonates Admitted with Sepsis to Neonatal Intensive Care Unit of Jimma Medical Center, A Tertiary Hospital in Ethiopia.
Berhane M, Gidi NW, Eshetu B, Gashaw M, Tesfaw G, Wieser A, Bårnes GK, Froeschl G, Ali S, Gudina EK.Ethiop J Health Sci. 2021 May;31(3):485-494. (IF 1.45)
A dried blood spot protocol for high throughput analysis of SARS-CoV-2 serology based on the Roche Elecsys anti-N assay.
Beyerl J*, Rubio-Acero R*, Castelletti N, Paunovic I, Kroidl I, Khan ZN, Bakuli A, Tautz A, Oft J, Hoelscher M, Wieser A; KoCo19 Study group .
EBioMedicine. 2021 Aug;70:103502. (IF 8,14)
Vaccine breakthrough infection and onward transmission of SARS-CoV-2 Beta (B.1.351) variant, Bavaria, Germany, February to March 2021.
Kroidl I, Mecklenburg I, Schneiderat P, Müller K, Girl P, Wölfel R, Sing A, Dangel A, Wieser A, Hoelscher M.
Euro Surveill. 2021 Jul;26(30):2100673. (IF 6.45)
In Search of the SARS-CoV-2 Protection Correlate: Head-to-Head Comparison of Two Quantitative S1 Assays in Pre-characterized Oligo-/Asymptomatic Patients.
Rubio-Acero R*, Castelletti N*, Fingerle V, Olbrich L, Bakuli A, Wölfel R, Girl P, Müller K, Jochum S, Strobl M, Hoelscher M, Wieser A; KoCo19 study team.
Infect Dis Ther. 2021 Sep;10(3):1505-1518. *: shared authorships (IF 4.31)
Broad T Cell Targeting of Structural Proteins After SARS-CoV-2 Infection: High Throughput Assessment of T Cell Reactivity Using an Automated Interferon Gamma Release Assay.
Brand I*, Gilberg L*, Bruger J*, Garí M, Wieser A, Eser TM, Frese J, Ahmed MIM, Rubio-Acero R, Guggenbuehl Noller JM, Castelletti N, Diekmannshemke J, Thiesbrummel S, Huynh D, Winter S, Kroidl I, Fuchs C, Hoelscher M*, Roider J*, Kobold S*, Pritsch M*, Geldmacher C*.
Front Immunol. 2021 May 20;12:688436. (*:shared authorships) (IF 5.09)
Evaluation of Adult Outpatient Antibiotics Use at Jimma Medical Center (with Defined Daily Doses for Usage Metrics).
Melaku T, Gashaw M, Chelkeba L, Berhane M, Bekele S, Lemi G, Wakjira T, Tesfaw G, Mekonnen Z, Ali S, Kroidl A, Wieser A, Froeschl G, Gudina EK.
Infect Drug Resist. 2021 Apr 28;14:1649-1658. (IF 3.84)
Antibiotic Resistance in Wastewater Treatment Plants and Transmission Risks for Employees and Residents: The Concept of the AWARE Study.
Wengenroth L, Berglund F, Blaak H, Chifiriuc MC, Flach CF, Pircalabioru GG, Larsson DGJ, Marutescu L, van Passel MWJ, Popa M, Radon K, de Roda Husman AM, Rodríguez-Molina D, Weinmann T, Wieser A, Schmitt H.
Antibiotics (Basel). 2021 Apr 21;10(5):478. (IF 3.89)
Prevalence and Risk Factors of Infection in the Representative COVID-19 Cohort Munich.
Pritsch M*, Radon K*, Bakuli A*, Le Gleut R*, Olbrich L, Guggenbüehl Noller JM, Saathoff E, Castelletti N, Garí M, Pütz P, Schälte Y, Frahnow T, Wölfel R, Rothe C, Pletschette M, Metaxa D, Forster F, Thiel V, Rieß F, Diefenbach MN, Fröschl G, Bruger J, Winter S, Frese J, Puchinger K, Brand I, Kroidl I, Hasenauer J*, Fuchs C*, Wieser A*, Hoelscher M*, On Behalf Of The KoCo Study Group.
Int J Environ Res Public Health. 2021 Mar 30;18(7):3572. (*: shared authorships) (IF 3.39)
Rapid prototyping vaccine approach in mice against multi-drug resistant Gram-negative organisms from clinical isolates based on outer membrane vesicles.
Pritsch M, Ben Khaled N, Liegl G, Schubert S, Hoelscher M, Woischke C, Arens N, Thorn-Seshold J, Kammermeier S, Wieser A.
Microbiol Immunol. 2021 May;65(5):214-227. (IF 1.95)
2020:
Regional Variation of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Enterobacterales, Fluoroquinolone-Resistant Salmonella enterica and Methicillin-Resistant Staphylococcus aureus Among Febrile Patients in Sub-Saharan Africa .
Moirongo RM, Lorenz E, Ntinginya NE, Dekker D, Fernandes J, Held J, Lamshöft M, Schaumburg F, Mangu C, Sudi L, Sie A, Souares A, Heinrich N, Wieser A, Mordmüller B, Owusu-Dabo E, Ayola Adegnika A, Coulibaly B, May J, Eibach D.
Front Microbiol. 2020; 11: 567235 .
Protocol of a population-based prospective COVID-19 cohort study Munich, Germany (KoCo19) .
Radon K, Saathoff E, Pritsch M, Guggenbühl Noller JM, Kroidl I, Olbrich L, Thiel V, Diefenbach M, Riess F, Forster F, Theis F, Wieser A, Hoelscher M, the KoCo19 collaboration group.
BMC Public Health. 2020; 20: 1036.
Publisher Correction to: Protocol of a population-based prospective COVID-19 cohort study Munich, Germany (KoCo19).
Radon K, Saathoff E, Pritsch M, Guggenbühl Noller JM, Kroidl I, Olbrich L, Thiel V, Diefenbach M, Riess F, Forster F, Theis F, Wieser A, Hoelscher M; KoCo19 collaboration group.
BMC Public Health. 2020 Sep 1; 20(1):1335.
Kupffer cell activation by different microbial lysates: Toll-like receptor-2 plays pivotal role on thromboxane A2 production in mice and humans.
Zhang J, Wieser A, Lin H, Li H, Hu M, Behrens IK, Schiergens TS, Gerbes AL, Steib CJ.
Eur J Immunol. 2020 Dec; 50(12):1988-1997.
Pretreatment with zinc protects Kupffer cells following administration of microbial products.
Zhang J, Wieser A, Lin H, Fan Y, Li H, Schiergens TS, Mayerle J, Gerbes AL, Steib CJ.
Biomed Pharmacother. 2020 May 14;127:110208
A Novel Rapid Sample Preparation Method for MALDI-TOF MS Permits Borrelia burgdorferi Sensu Lato Species and Isolate Differentiation.
Neumann-Cip AC, Fingerle V, Margos G, Straubinger RK, Overzier E, Ulrich S, Wieser A.
Front Microbiol. 2020 Apr 21;11:690.
Heteroresistant Bacteria Detected by an Extended Raman-based Antibiotic Susceptibility Test.
Bauer D, Wieland K, Qiu L, Neumann-Cip AC, Magistro G, Stief CG, Wieser A*, Haisch C.*
Anal Chem. 2020 Apr 14.
2019:
Patients with cirrhosis and SBP: increase in multidrug-resistant organisms and complications.
Li H*, Wieser A*, Zhang J, Liss I, Markwardt D, Hornung R, Neumann-Cip AC, Mayerle J, Gerbes AL, Steib CJ.
Eur J Clin Invest. Epub 2019 Dec 30.
Reply to comment: Empirical antibiotic therapy for patients with SBP and ACLF - Need for rapid decision.
Wieser A*, Li H*, Suerbaum S, Mayerle J, Gerbes AL, Steib CJ.
Dig Liver. Epub 2019 Nov 14.
Yeast cell wall - Silver nanoparticles interaction: A synergistic approach between surface-enhanced Raman scattering and computational spectroscopy tools.
Gherman AMR, Dina NE, Chiș V, Wieser A, Haisch C.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 May 31.
Evaluating the best empirical antibiotic therapy in patients with acute-on-chronic liver failure and spontaneous bacterial peritonitis.
Wieser A*, Li H*, Zhang J, Liss I, Markwardt D, Hornung R, Suerbaum S, Mayerle J, Gerbes AL, Steib CJ.
Dig Liver Dis. Epub 2019 Mar 31.
2018:
Identifying dormant growth state of mycobacteria by orthogonal analytical approaches on a single cell and ensemble basis.
Neumann AC, Bauer D, Hölscher M, Haisch C, Wieser A.
Anal Chem. 2018 Dec 3. (IF 6.3 (2016))
Emergence of high drug resistant bacterial isolates from patients with health care associated infections at Jimma University medical center: a cross sectional study.
Gashaw M, Berhane M, Bekele S, Kibru G, Teshager L, Yilma Y, Ahmed Y, Fentahun N, Assefa H, Wieser A, Gudina EK, Ali S.
Antimicrob Resist Infect Control. 2018 Nov 19;7:138. eCollection 2018.
Prevalence and antibiotic susceptibility pattern of CTX-M type extended-spectrum β-lactamases among clinical isolates of gram-negative bacilli in Jimma, Ethiopia.
Zeynudin A*, Pritsch M*, Schubert S, Messerer M, Liegl G, Hoelscher M, Belachew T, Wieser A. (*: shared first authorship)
BMC Infect Dis. 2018 Oct 20;18(1):524.
Microcystin-LR Enrichment from Freshwater by a Recombinant Plant-Derived Antibody Using Sol-Gel-Glass Immunoextraction.
Neumann AC, Melnik S, Niessner R, Stoeger E, Knopp D.
Anal Sci. 2018 Oct 12.
Comparison of the bactericidal effect of cold atmospheric pressure plasma (CAPP), antimicrobial photodynamic therapy (aPDT), and polihexanide (PHX) in a novel wet surface model to mimic oral cavity application.
Hafner S, Ehrenfeld M, Neumann AC, Wieser A.
JCraniomaxillofac Surg. 2018 Sep 19.
Glucose-6-phosphate dehydrogenase activity measured by spectrophotometry and associated genetic variants from the Oromiya zone, Ethiopia.
Kiessling N, Brintrup J, Zeynudin A, Abduselam N, Götz S, Mack M, Pritsch M, Wieser A, Kohne E, Berens-Riha N.
Malar J. 2018 Oct 12;17(1):358.
Outcome of patients with acute bacterial meningitis in a teaching hospital in Ethiopia: A prospective study.
Gudina EK, Tesfaye M, Wieser A, Pfister HW, Klein M.
PLoS One. 2018 Jul 18;13(7):e0200067.
Relapsing fever caused by Borrelia - disease and diagnostic challenge .
A. Wieser, V. Fingerle
Review paper - Der Mikrobiologe March 2018
Cloning and plant-based production of antibody MC10E7 for a lateral flow immunoassay to detect [4-arginine]microcystin in freshwater.
Melnik S, Neumann AC, Karongo R, Dirndorfer S, Stübler M, Ibl V, Niessner R, Knopp D, Stoger E.
Plant Biotechnol J. 2018 Jan;16(1):27-38.
Characterization of Clinically Relevant Fungi via SERS Fingerprinting Assisted by Novel Chemometric Models.
Dina NE, Gherman AMR, Chiş V, Sârbu C, Wieser A, Bauer D, Haisch C.
Anal Chem. 2018 Feb 20;90(4):2484-2492.
2017:
First insights into the variability of Borrelia recurrentis genomes.
Marosevic D, Margos G, Wallich R, Wieser A, Sing A, Fingerle V.,
PLoS Negl Trop Dis. 2017 Sep 13;11(9):e0005865. (IF 7.64 (2015))
The small liver fluke (Dicrocoelium dendriticum): an unusual clinical finding in chronic urticarial.
Blatner C, Oppel E M, Wieser A, Herzinger T, Ruëff F.
Allergo Journal International (IF 1 (2015))
New microbiological techniques .
S. Schubert, A. Wieser, G. Bonkat
Urologist56, 711-719 (2017).
First report on bla NDM-1-producing Acinetobacter baumannii in three clinical isolates from Ethiopia.
Pritsch M*, Zeynudin A*, Messerer M, Baumer S, Liegl G, Schubert S, Löscher T, Hoelscher M, Belachew T, Rachow A, Wieser A.
BMC Infect Dis. 2017 Mar 1;17 (IF 2.82 (2015))
Analysis of the Virulence of Uropathogenic Escherichia coli Strain CFT073 in the Murine Urinary Tract.
Waldhuber, A., Puthia, M., Wieser, A., Svanborg, C. and Miethke, T.
Bio-protocol 7(3): e2129 (2017).
2016:
Current situation of lice relapse fever with special consideration of asylum seekers.
Fingerle F, Ackermann N, Belting A, Hoch M, Liebl B, Margos G, Pürner F, Schönberger K, Wieser A, Sing A.
Hyg Med. 2016; 41(11)
Strain-specific impact of the high-pathogenicity island on virulence in extra-intestinal pathogenic Escherichia coli. Smati M, Magistro G, Adiba S, Wieser A, Picard B, Schubert S, Denamur E.
Int J Med Microbiol. 2016 Nov 22 (IF 3,898 (2015))
Drug resistance and population structure of M. tuberculosis isolates from prisons and communities in Ethiopia.
Ali S, Beckert P, Haileamlak A, Wieser A, Pritsch M, Heinrich N, Loescher T, Hoelscher M, Niemann S, Rachow A. BMC Infectious Dis. 2016 Nov 21;16(1):687
Similar trends of susceptibility in Anopheles arabiensis and Anopheles pharoensis to Plasmodium vivax infection in Ethiopia.
Abduselam N, Zeynudin A, Berens-Riha N, Seyoum D, Pritsch M, Tibebu H, Eba K, Hoelscher M, Wieser A*, Yewhalaw D.* (*last authors contributed equally, correspondence Wieser A.)
Parasites and Vectors, 2016 Oct 18;9(1):552. (IF 3.32 (2014))
Adjunctive dexamethasone therapy in unconfirmed bacterial meningitis in resource limited settings: is it a risk worth taking?
Gudina E K, Tesfaye M, Adane A, Kinfe L, Shibiru T, Wieser A, Pfister H-W, Klein M.
BMC Neurology 2016 Aug 26;16(1):153. (IF 1,961 (2015))
Louse-Borne-Relapsing Fever in refugees from the Horn of Africa; a case series of 25 patients.
Seilmaier M, Guggemos W, Wieser A, Fingerle V, Balzer L, Fenzl T, Hoch M, von Both U, Schmidt HU, Wendtner CM, Strobel E.
DMW (2016) (IF 0.503 (2015))
Relapsing fever: an almost forgotten disease in focus again.
Wieser A, Löscher T, Schunk M, Seilmaier M, Balzer L, Margos G, von Both U, Schulzki T, Kopf S, Hoch M, Sing A, Fingerle V.
Deutsche Medizinische Wochenschrift (DMW), 2016 Jul; 141(14). (IF 0.542 (2014))
The uropathogenic Escherichia coli strain CFT073 disrupts the inflammasome activation.
Waldhuber A, Cirl C, Dürr S, Neumann-Pfeifer S, Albrecht S, Römmler F, Müller T, Zheng Y, Schubert S, Groß O, Svanborg C, Miethke T.
JCLIN INVEST 2016 Jul 1;126(7):2425-36 (IF 13.22 (2014))
"Candidatus Borrelia kalaharica" detected from a febrile traveler returning to Germany from vacation in Southern Africa.
Fingerle V*, Pritsch M*, Wächtler M, Margos G, Ruske S, Jung J, Löscher T, Wendtner C, Wieser A (*first authors contributed equally)
PLOS Negl Trop Dis. 2016 Mar 31;10(3):e0004559. (IF 4.45 (2014))
Photodynamic Inactivation of Actinomyces naeslundii in Comparison with Chlorhexidine and Polihexanide - a new approach for antiseptic treatment of MRONJ?
Hafner S, Storz E, Wieser A.
Journal of Oral and Maxillofacial Surgery (JOMS), 2016 Mar;74(3):516-22; (IF 1.43 (2014))
"Comparison of Intranasal Outer Membrane Vesicles with Cholera Toxin and Injected MF59C.1 as Adjuvants for Malaria Transmission Blocking Antigens AnAPN1 and Pfs48/45.
Pritsch M, Ben-Khaled N, Chaloupka M, Kobold S, Berens-Riha N, Peter A, Liegl G, Schubert S, Hoelscher M, Loscher T, Wieser A.
Journal of Immunology Research 2016; 2016:3576028. (IF 2.93 (2014))
2015:
Louse-borne relapsing fever (Borrelia recurrentis) diagnosed in 15 refugees from northeast Africa: epidemiology and preventive control measures, Bavaria, Germany, July to October 2015
Hoch M, Wieser A, Löscher T, Margos G, Pürner F, Zühl J, Seilmaier M, Balzer L, Guggemos W, Rack-Hoch A, von Both U, Hauptvogel K, Schönberger K, Hautmann W, Sing A, Fingerle V.
Eurosurveillance (2015), Volume 20, Issue 42, October 22, 2015. (IF 5.7 (2014))
Primary skin melioidosis in a returning traveler.
Huber K, Thoma B, Löscher T, Wieser A.
Infection. 2015 Aug;43(4):507-8. (IF 2.8)
Label-Free In-Situ Discrimination of Live and Dead Bacteria by Surface-Enhanced Raman Scattering.
Zhou H, Yang D, Ivleva NP, Mircescu NE, Schubert S, Niessner R, Wieser A*, Haisch C*; (*contributed equally); Analytical Chemistry 2015, Jul; 7; 87(13):6553-61 (IF 5.83 (2014))
Prevalence of pulmonary tuberculosis among prison inmates in Ethiopia, a cross-sectional study.
Ali S, Haileamlak A, Wieser A, Pritsch M, Heinrich N, Loescher T, Hoelscher M, Rachow A.
PLoS ONE (2015). Volume 10, Issue 12 (IF 3.23 (2014))
Surface-enhanced Raman scattering detection of bacteria on microarrays at single cell levels using silver nanoparticles.
Zhou H, Yang D, Mircescu NE, Ivleva NP, Schwarzmeier K, Wieser A, Schubert S, Niessner R, Haisch C.
Microchimica Acta. (2015) (IF 3,74)
Molecular markers of anti-malarial drug resistance in southwest Ethiopia over time: regional surveillance from 2006 to 2013.
Heuchert A, Abduselam N, Zeynudin A, Eshetu T, Löscher T, Wieser A, Pritsch M, Berens-Riha N;
Malaria J. (2015) 14:208. (IF 3.49)
Efficacy and safety of triple combination therapy with artesunate-amodiaquine-methylene blue for falciparum malaria in children: a randomized controlled trial in Burkina Faso.
Coulibaly B, Pritsch M, Bountogo M, Meissner PE, Nebié E, Klose C, Kieser M, Berens-Riha N, Wieser A, Sirima SB, Breitkreutz J, Schirmer RH, Sié A, Mockenhaupt FP, Drakeley C, Bousema T, Müller O.
JInfect Dis. 2015 Mar 1; 211(5):689-97. (IF 5.78)
2014:
Efficient quantification and characterization of bacterial outer membrane derived nano-particles with flow cytometric analysis.
Wieser A, Storz E, Liegl G, Peter A, Pritsch M, Shock J, Wai SN, Schubert S.
Int J Med Microbiol. 2014 Nov; 304(8):1032-7. (IF 3.42)
Bacteriophage-based latex agglutination test for rapid identification of Staphylococcus aureus.
Idelevich EA, Walther T, Molinaro S, Li X, Xia G, Wieser A, Peters G, Peschel A, Becker K.
JClin Microbiol. 2014 Sep; 52(9):3394-8. Epub 2014 Jul 16. (IF 4.23)
Corticosteroid-induced meningococcal meningitis in a patient with chronic meningococcemia.
Wenzel M, Jakob L, Wieser A, Schauber J, Dimitriadis K, Schubert S, Pfister HW.
JAMA Dermatol. 2014 Jul; 150(7):752-5. (IF 4.3).
Rapid detection of antibiotic resistance based on mass spectrometry and stable isotopes.
Jung JS, Eberl T, Sparbier K, Lange C, Kostrzewa M, Schubert S*, Wieser A*; (*contributed equally);
Eur J Clin Microbiol Infect Dis. 2014 Jun; 33(6):949-55. (IF 2.54)
Towards a receptor-free immobilization and SERS detection of urinary tract infections causative pathogens.
Mircescu NE, Zhou H, Leopold N, Chiş V, Ivleva NP, Niessner R, Wieser A, Haisch C.
Anal Bioanal Chem. 2014 May; 406(13):3051-8. (IF 3.58)
TcpC protein from E. coli Nissle improves epithelial barrier function involving PKCζ and ERK1/2 signaling in HT-29/B6 cells.
Hering NA, Richter JF, Fromm A, Wieser A, Hartmann S, Günzel D, Bücker R, Fromm M, Schulzke JD, Troeger H. Mucosal Immunol. 2014 Mar; 7(2):369-78. (IF 7.54)
2013:
Molecular analysis of a novel Toll/interleukin-1 receptor (TIR)-domain containing virulence protein of Y. pseudotuberculosis among Far East scarlet-like fever serotype I strains.
Nörenberg D, Wieser A, Magistro G, Hoffmann C, Meyer C, Messerer M, Schubert S.
Int J Med Microbiol. 2013 Dec; 303(8):583-94. (IF 3.42)
Evaluation of Plasmodium falciparum gametocyte detection in different patient material.
Kast K, Berens-Riha N, Zeynudin A, Abduselam N, Eshetu T, Löscher T, Wieser A, Shock J, Pritsch M.
Malar J. 2013 Dec 4; 12:438. (IF 3.49)
MALDI biotyper-based rapid resistance detection by stable-isotope labeling.
Sparbier K, Lange C, Jung J, Wieser A, Schubert S, Kostrzewa M.
JClin Microbiol. 2013 Nov; 51(11):3741-8. (IF 4.23)
Triple worm infestation in an HIV-infected patient.
Sammet S, Wieser A, Müller S, Huber M, Schubert S, Seybold U.
Infection. 2013 Oct; 41(5):1053-4. (IF 2.86)
2012:
Open-label trial with artemether-lumefantrine against uncomplicated Plasmodium falciparum malaria three years after its broad introduction in Jimma Zone, Ethiopia.
Eshetu T, Abdo N, Bedru KH, Fekadu S, Wieser A, Pritsch M, Löscher T, Berens-Riha N.
Malar J. 2012 Jul 23; 11:240. (IF 3.4)
Salmonella transiently reside in luminal neutrophils in the inflamed gut.
Loetscher Y, Wieser A, Lengefeld J, Kaiser P, Schubert S, Heikenwalder M, Hardt WD, Stecher B.
PLoS One. 2012; 7(4):e34812. (IF 3.73)
Stability of gametocyte-specific Pfs25 mRNA in dried blood spots on filter paper subjected to different storage conditions.
Pritsch M*, Wieser A*, Soederstroem V, Poluda D, Eshetu T, Hoelscher M, Schubert S, Shock J, Loescher T, Berens-Riha N.(*contributed equally);
Malar J. 2012 Apr 30; 11:138. (IF 3.40)
A Novel Method for the Preparation of a Strain-Adapted Vaccine.
Schubert S. 50%, Wieser A. 50% LMU patent application PCT/EP2013/057216 filed on April 5, 2012
First multi-epitope subunit vaccine against extraintestinal pathogenic Escherichia coli delivered by a bacterial type-3 secretion system (T3SS).
Wieser A, Magistro G, Nörenberg D, Hoffmann C, Schubert S.
Int J Med Microbiol. 2012 Jan; 302(1):10-8. (IF 4.54)
2011:
Novel, improved sample preparation for rapid, direct identification from positive blood cultures using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry.
Schubert S, Weinert K, Wagner C, Gunzl B, Wieser A, Maier T, Kostrzewa M.
JMol Diagn. 2011 Nov; 13(6):701-6. (IF 3.58)
Intra- and extracellular biofilms of uropathogenic E. coli - significance for the pathogenesis of urinary tract infection.
Wieser A, Schubert S.
Chemother J 2011; 20:181-5 .
Use of matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) in routine microbiological diagnostics.
Schubert S, Wieser A.
JLab Med 2011; 35(4):195
A novel ex vivo set-up for dynamic long-term characterization of processes on mucosal interfaces by confocal imaging and simultaneous cytokine measurements.
Wieser A*, Guggenberger C*, Pritsch M, Heesemann J, Schubert S. (*contributed equally).
Cell Microbiol. 2011 May; 13(5):742-51. (IF 5.46)
MALDI-TOF MS in microbiological diagnostics-identification of microorganisms and beyond (mini review).
Wieser A, Schneider L, Jung J, Schubert S.
Appl Microbiol Biotechnol. 2012 Feb; 93(3):965-74. Epub 2011 Dec 25.
2010:
MALDI-TOF MS in microbiological diagnostics.
Wieser A, Schubert S.
Biospektrum 07.10 (760-762), 16th year
Prevalence and phylogenetic history of the TcpC virulence determinant in Escherichia coli.
Schubert S, Nörenberg D, Clermont O, Magistro G, Wieser A, Romann E, Hoffmann C, Weinert K, Denamur E.
Int J Med Microbiol. 2010 Nov; 300(7):429-34. (IF 2.40)
A multiepitope subunit vaccine conveys protection against extraintestinal pathogenic Escherichia coli in mice.
Wieser A, Romann E, Magistro G, Hoffmann C, Nörenberg D, Weinert K, Schubert S.
Infect Immun. 2010 Aug; 78(8):3432-42. (IF 4.10)
2009:
Role of intraspecies recombination in the spread of pathogenicity islands within the Escherichia coli species.
Schubert S, Darlu P, Clermont O, Wieser A, Magistro G, Hoffmann C, Weinert K, Tenaillon O, Matic I, Denamur E.
PLoS Pathog. 2009 Jan; 5(1):e1000257. (IF 8.98)
2008:
Subversion of Toll-like receptor signaling by a unique family of bacterial Toll/interleukin-1 receptor domain-containing proteins.
Cirl C, Wieser A, Yadav M, Duerr S, Schubert S, Fischer H, Stappert D, Wantia N, Rodriguez N, Wagner H, Svanborg C, Miethke T.
Nat Med. 2008 Apr; 14(4):399-406. (IF 27.55)
