B2

B2: Genetically modified pigs to modulate vascular and cellular rejection

We have produced single loci multi-modified pigs to combat hyperacute and vascular rejection. Our goal is now to improve long-term xenograft survival. We will a) define the multi-transgene integration site and determine PERV load; b) characterise transgene expression patterns in detail and assess the survival and functionality of multi-modified porcine tissues and organs as xenografts; c) introduce additional modifications as necessary; d) develop a “smart graft” strategy to express immuno-regulatory and tissue-protective